实验研究-救顽汤对慢性湿疹模型大鼠作用研究

[摘要]：目的：通过观察救顽汤对慢性湿疹大鼠模型皮损、脾脏指数，初步评价救顽汤治疗慢性湿疹作用。方法：随机将60只雄性SD大鼠分为6组，每组10只，分别为空白组、模型组、阳性组、救顽汤低剂量组、中剂量组、高剂量组。除空白组外，各组大鼠运用2,4-二硝基氯苯（DNCB）复制慢性湿疹动物模型。模型建立后连续给药10天，比较各组间皮损程度、脾指数。结果：与空白组相比较，模型组大鼠皮损评分显著升高，脾脏指数降低(P<0.01)；与模型组相比较，救顽汤高剂量、中剂量组给药后皮损评分明显降低，脾脏指数升高 (P<0.01)，低剂量组无差异(P>0.05)。结论：救顽汤能有效减轻慢性湿疹大鼠模型的皮损程度，降低皮损评分，具有明显的治疗作用。救顽汤能有效升高湿疹模型降低的脾指数，其治疗慢性湿疹的作用机制可能与提高机体免疫有关。

基金项目：黔南民族医学高等专科学校科研基金（项目编号：QNYZ201830）

【Abstract】: Objective To observe the effects of Jiuwan Decoction on skin lesions, spleen in rats model of chronic eczema, the model was established to initially investigate the immune mechanism of Jiuwan Decoction in curing chronic eczema. Method 60 SD rats were randomly divided into 6 groups including blank group, model group, positive group, low-dose, mid-dose and high-dose groups with 10 rats in each group. The test groups were used to replicate the animal model of chronic eczema using 2,4-dinitrochlorobenzene (DNCB) except for the blank group. These rats were doses for 10 days after successful model establishment. The degree of skin lesions and spleen index were compared among these groups. Results Compared with the blank group, the skin lesion score of the model group increased statistically whereas the spleen index decreased significantly (P<0.01 ). Compared with the model group, the skin lesion score level in the high- and mid-dose groups of Jiuwan Decoction decreased significantly, and the spleen index content increased (P<0.01 ). There was no difference in the low dose group (P>0.05).Conclusion JIUWAN Decoction can effectively reduce the degree of skin lesions in the rat model of chronic eczema, reduce the score of skin lesions, and has an obvious therapeutic effect. JIUWAN Decoction can effectively increase the spleen index reduced in the eczema model, and its therapeutic mechanism for chronic eczema may be related to the improvement of body immunity.

慢性湿疹是一种临床常见以炎性为主的慢性疾病，研究认为其发病机制是由T细胞所介导的Ⅳ型变态反应，其病因可能是遗传、免疫损伤、细胞凋亡以及环境因素等共同作用的结果[1]。由于慢性湿疹的发病机制较为复杂，在免疫损伤方面，研究发现该病发生的重要免疫学因素之一即TH1/TH2 失衡所致[2]。救顽汤出自清代医家陈士铎所著[3]《辨证录》第735条：“人有久生恶疮，或在手足，或在胸背，或在头面，终年经岁而不愈，世人谓之顽疮，救顽汤治之。”该方为中和医派杨建宇教授治疗慢性湿疹的常用方。本实验拟通过建立大鼠慢性湿疹模型，观察救顽汤对慢性湿疹大鼠模型皮损、脾指数的影响，初步探讨救顽汤治疗慢性湿疹的作用。

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救顽汤对慢性湿疹模型大鼠作用研究.pdf