实验研究-复方米蒿籽软膏剂治疗烧烫伤的药效学实验研究

[摘要]  目的：通过抗炎、镇痛、抑菌、水肿抑制实验及组织学观察等实验，观察复方米蒿籽软膏剂对烧烫伤的治疗作用。方法：分别设阴性对照组、阳性对照组、高剂量组和低剂量组，采用二甲苯致小鼠耳廓肿胀试验、热板法、琼脂平板扩散法和水肿抑制作用实验，进行复方米蒿籽软膏剂对小鼠Ⅱ度烫伤的药效学评价。结果：复方米蒿籽软膏高低剂量均对二甲苯致小鼠耳廓肿胀有抑制作用，且低剂量组对肿胀的抑制程度接近于阳性对照组，具有显著抗炎作用（p=0.0023）；该制剂高剂量组还具有缓解热板性疼痛的效应（p=0.0365）；并具有较好的抑制小鼠烫伤性水肿的作用（p=0.0390）；尚可抑制葡萄球菌、大肠埃希菌为代表的G+细菌和G-细菌生长的作用。组织病理切片观察，用该制剂7天，皮肤组织伴有成纤维细胞生成，深层结构汗腺、毛囊腺恢复，表皮层次结构日趋完整，对试验性烧烫伤的治疗作用显著。结论：复方米蒿籽软膏具有显著治疗实验动物烧烫伤创面的作用。

[Abstract]  Objective: To observe the effects of Compound Mihao seeds Ointment on burn and scald by using anti-inflammatory, analgesic, bacteriostasis, edema inhibition, and histological staining assays.  Method: The mice were used in this study, and separated into 4 groups including negative control, positive control, high-dose, and low-dose group. The pharmacodynamics of Compound Mihao seeds Ointment on second degree scald in mice was evaluated by Xylene-induced auricular swelling test, hot plate method, agar plate diffusion method, and edema inhibition test. Result: The high and low doses of Compound Mihao seeds Ointment inhibited auricular swelling induced by xylene in mice, and the low dosage of the ointment, with significant anti-inflammatory effect (p=0.0023), inhibited auricle swelling similar to the positive control group . The high dosage of ointment had the effect of relieving hot-plate pain (p=0.0365), and also had a better effect of inhibiting scalded edema in mice (p=0.0390). Moreover, the ointment inhibited the growth of Staphylococcus (G + bacteria) and Escherichia coli. (G – bacteria). Histologically, in the mouse skin where treated with the ointment for 7 days, the fibroblasts were formed, sweat glands and follicular glands in deep skin structure were restored, and epidermis layers were becoming more and more complete. These findings indicated that the ointment had a significant therapeutic effect on experimental burn and scald. Conclusion: Compound Mihao seeds Ointment has significant therapeutic effect on burn and scald wounds in experimental mice.

烧烫伤是一种治疗周期长，且极易引起并发症的疾病，如不及时和有效的治疗，会导致烧烫伤创面难以修复，留下不能消退的瘢痕，更甚者会导致烧烫伤患者死亡。我们已研究开发的治疗烧烫伤的复方米蒿籽软膏，通过对小鼠烧烫伤皮肤组织切片检测，其在消除水肿、迅速结痂、促进创面愈合等方面具有良好效果。为了进一步探明软膏治疗烧烫伤的作用机制，我们对复方米蒿籽软膏进行了药效学研究，客观评价复方米蒿籽软膏对烧烫伤的治疗效果。

[参考文献]

[1]  赵媛，万凯华，翁幼武，等．紫草三黄栓抗炎、消肿、镇痛药效作用的研究[J]．新疆医科大学学报，2018，41(7)：883-885．

[2]  王南舟．蜂胶制剂镇痛抗炎作用初探[J]．蜜蜂杂志，2004，5：3-6．

[3]  向双云，周珍辉，李玉冰，等．8种中草药对大肠杆菌的体外抑菌试验[J]．黑龙江畜牧兽医，2016，5：167-169．

[4]  温志鹏，曾小粤，刘心亮，等．烧伤方对实验小鼠烫伤致水肿的抑制作用研究[J]．中国中医急症，2007，16（11）：1382，1385．

[5]  李杏娟．病理技术HE染色在病理诊断中的应用研究[J]．现代医学与健康研究电子杂志，2018，2(16)：166，168．

[6]  沈娟，金小宝，丁静，等．烧烫伤创面感染的小鼠模型构建[J]．中国实验动物学报，2013，21(3)：65-69．

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