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实验研究-胃香乐方对功能性消化不良大鼠胃动素及胃窦Cajal间质细胞 超微结构的影响

摘  要:目的:观察胃香乐方对功能性消化不良(functional dyspepsia,FD)模型大鼠的防治效果并探讨其机理。方法:60只大鼠随机分为模型组,胃香乐方高、中、低剂量组,莫沙必利对照组和空白组。采用复合病因法复制FD模型,分别以相应药物灌胃14d,计算胃排空率、小肠推进率,测定血浆胃动素(motilin,MTL)水平,电镜下观察胃窦Cajal间质细胞超微结构并进行定量分析。结果:莫沙必利组和胃香乐方各组胃排空率、小肠推进率均显著高于模型组(P<0.05),尤以中剂量组和莫沙必利组效果最好;与模型组相比,莫沙必利组和胃香乐方各组MTL均明显升高(P<0.01,P<0.05),尤以中剂量组效果最佳;与模型组比较,莫沙必利组和胃香乐方各组大鼠胃窦Cajal间质细胞超微结构均明显改善;与模型组及莫沙必利组比较,胃香乐方各组线粒体数量和Vv、δ、δm均明显增加(P<0.01,P<0.05)。结论:胃香乐方具有促进胃肠动力、防治功能性消化不良的作用。


Abstract:Objective: To explore the preventive and therapeutic effect and mechanisms of Weixiangle Prescription(WXP)on functional dyspepsia rats. Methods: 60 rats were randomly divided into model group, big-dosage, medium-dosage and small-dosage group of WXP, mosapride group and control group. Each was respectively perfused with corresponding drug for 14 days after the functional dyspepsia models were duplicated by complex etiological method. The gastric emptying rate and the small intestine propulsion rate were calculated, the motilin level of blood plasma was measured, the ultrastructure of gastric antrum Cajal interstitial cells was observed under electric microscope, and quantitative analysis of which were carried out. Results: Compared with control group, the gastric emptying rate and the small intestine propulsion rate of mosapride group and each WXP group increased obviously(P<0.05), especially the medium-dosage group and mosapride group. Compared with model group, the MTL level of mosapride group and each WXP group significantly increased(P<0.01 or P<0.05), especially the medium-dosage group. Compared with control group, the gastric antrum Cajal interstitial cells ultrastructure of mosapride group and each WXP group were obviously improved. Compared with control group and mosapride group, the number of mitochondria and Vv, δ, δm of each WXP group increased obviously(P<0.01 or P<0.05). Conclusion: WXP has the effect of promoting gastrointestinal motility and treating functional dyspepsia. 


FD是临床常见的消化系疾病之一。近年来,其发病率呈逐年升高趋势。现代医学认为,本病主要系因胃肠运动障碍所致,治疗主要采用促胃肠动力等药物,但其作用单一,疗效不稳定,且易复发,副反应多[1]。胃香乐方(由鸡内金、砂仁、黄连、吴茱萸、莱菔子、槟榔、公丁香、黑米等组成)是首届全国名中医、河南中医药大学第二附属医院毛德西教授治疗FD的基本方,经长期临床观察疗效显著。本文在既往研究的基础上[2],观察了胃香乐方对FD模型大鼠胃排空率、小肠推动率、胃动素、胃窦Cajal间质细胞超微结构等的影响。


参考文献:

[1]邵长乐,陈婉珍,朱方石. 中医治疗功能性消化不良的疗效机制研究[J]. 四川中医,2019,37(8):189~192.

[2]毛德西. 毛德西方药心悟[M]. 北京:人民卫生出版社,2015:138~140.

[3]Ghoshal UC, Singh R, Chang FY, et al. Epidemiology of uninvestigated and functional dyspepsia in Asia:facts and fiction[J]. J Neurogastroenterol Motil, 2011(17):234-244. 

[4]欧阳华,姜红建,李强. 功能性消化不良的抗抑郁焦虑药物治疗研究[J]. 山西医药杂志,2019,48(21):2634~2635.

[5]刘永林,黄文豪. 112 例功能性消化不良患者的心理状况分析[J]. 医学理论与实践,2014,27(10):1313~1314.

[6]张声生,赵鲁卿. 功能性消化不良中医诊疗专家共识意见(2017)[S]. 中华中医药杂志,2017,32(6):2595~2598.

[7]韦德锋,韦维,刘熙荣,等. 情志因素影响功能性消化不良的临床研究概况[J]. 湖南中医杂志,2018,34(11):174~176.

[8]刘俊宏,汪龙德,毛兰芳,等. 功能性消化不良中医药研究进展[J]. 甘肃中医药大学学报,2017,34(6):91~94.


 胃香乐方对功能性消化不良大鼠胃动素及胃窦Cajal间质细胞 超微结构的影响.pdf


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