实验研究-基于BGP、TGF-β1的表达探究蛇床子素对去卵巢致骨质疏松大鼠的作用

    摘要：目的：观察蛇床子素对去卵巢致骨质疏松大鼠的影响。方法：选取3月龄雌性SD大鼠30只，随机分为蛇床子素组（A组）、模型对照组（B组）、假手术组（C组），各10只。A、B两组摘除卵巢构建去势大鼠骨质疏松模型，C组仅进行手术、不摘除卵巢。造模成功后，分别给予相应药物灌胃，连续给药12周，处死。然后测量骨密度，检测治疗后血清BGP、TGF-β1、钙指标。结果：B组大鼠股骨骨密度较C组明显降低( P＜0.05)；经12周治疗，A组股骨骨密度较B组明显升高( P＜0.05)。与C组比较，B组大鼠血清中BGP含量升高、TGF-β1降低(P＜0.05)；与B组比较，A组大鼠血清中BGP降低、TGF-β1含量升高( P＜0.05)。与C组比较，B组大鼠血清Ca水平明显降低( P＜0.05)；与B组比较，A组大鼠血清Ca水平明显升高（P＜0.05）。差异均有统计学意义。结论：蛇床子素能够有效通过调节大鼠体内激素分泌改善去卵巢大鼠的骨代谢异常，提高骨密度，对骨质疏松症起到一定的防治作用。

   Abstract: Objective: To observe the effect of Osthole on ovariectomized osteoporosis rats. Methods: Thirty 3-month-old female SD rats were randomly divided into Osthole group (group A), model control group (group B) and sham operation group (group C), with 10 rats in each group. Ovariectomy was performed in group A and B to establish osteoporosis model in ovariectomized rats. In group C, ovariectomy was performed without ovariectomy. After successful modeling, the rats were given corresponding drugs by gavage for 12 weeks, and then executed. Bone mineral density (BMD) was measured and serum BGP, TGF-β1 and calcium were measured after treatment. Results: Bone mineral density of femur in group B was significantly lower than that in group C (P < 0.05); after 12 weeks of treatment, bone mineral density of femur in group A was significantly higher than that in group B (P < 0.05). Compared with group C, serum BGP and TGF-β1 levels in group B were higher and lower (P < 0.05); compared with group B, serum BGP and TGF-β1 levels in group A were lower and higher (P < 0.05). Compared with group C, serum Ca level in group B was significantly lower (P < 0.05), while that in group A was significantly higher (P < 0.05). The differences were statistically significant. Conclusion: Osthole can effectively improve the abnormal bone metabolism and bone mineral density of ovariectomized rats by regulating hormone secretion in vivo, and play a role in preventing and treating osteoporosis.

   骨质疏松症（Osteoporosis，OP）是一种以骨量低下，骨微结构损坏，导致骨脆性增加，易发生骨折为特征的全身性骨病[1]。其产生原因与骨重建过程中成骨细胞介导的骨生成和破骨细胞介导的骨吸收之间的平衡失调有关[2]。研究表明，卵巢激素分泌不足，雌激素水平下降是该群体骨质疏松表现的重要原因[3]。现人均寿命的提高，老龄化社会的到来，骨质疏松症人数逐年增加。绝经后骨质疏松症（Postmenopausal Osteoporosis，PMOP）属于原发性骨质疏松，绝经后老年妇女的一种常见病、多发病，严重威胁着老年人的健康。伴随世界人口老龄化的发展，如何防治OP现成为国际学术界关注的焦点。因此，从根源上寻找它的发病机制，制定有效的防治策略，提供充分的理论依据都十分重要。中医药在骨质疏松防治方面具有一定优势，毒副作用小，可以长期服用，有化学药品无法取代的优势，其治法多种多样，有补肾、健脾等，骨质疏松属于中医“骨痿”范畴，其中医病机主要责之于肾虚。本实验使用去卵巢致骨质疏松大鼠模型，使用蛇床子素进行干预，观察骨密度、血清BGP、TGF-β1、血清钙等相关指标，以求证蛇床子素具有一定抗骨质疏松作用，并探究其作用的可能机制，为早期预防骨质疏松及其并发症，提供一种安全性高、效果良好的中药。

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