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实验研究-白虎加人参汤对气阴两虚型糖尿病模型大鼠肺功能的影响

   摘要:目的 探讨白虎加人参汤对气阴两虚型糖尿病(DM)大鼠肺功能的影响。 方法  将60只SPF级SD系6周龄雄性大鼠随机抽取6只为对照组,余54只通过高脂高糖饲养联合一次性腹腔注射链脲佐菌素(STZ)30mg/kg溶解于0.1 mol/L灭菌枸橼酸钠缓冲液(pH 4.0)制备DM大鼠模型。筛选符合气阴两虚型的糖尿病模型大鼠30只,随机分为5组,即模型组、白虎加人参汤高、中、低剂量组、二甲双胍组,每组6只。白虎加人参汤高、中、低剂量组分别予27.5g•kg-1•d-1、13.8g•kg-1•d-1、6.9g•kg-1•d-1的白虎加人参汤煎液进行灌胃,连续给药3周。干预3周后,观察不同剂量的白虎加人参汤对气阴两虚型DM模型大鼠的血糖、体重、一般情况及肺功能参数的影响。结果 与模型组比较,白虎加人参汤高、中、低剂量均可改善DM模型大鼠的用力肺活量(FVC),呼吸后期瞬间流速(FEF25%或V25),吸气峰流速(PIF)等肺功能参数。 结论 白虎加人参汤可降低DM模型大鼠的血糖,对气阴两虚型DM大鼠的肺功能有显著的改善作用,以高剂量效果最为显著。


   [Abstract] Objective: Investigate the effect of baihu and ginseng decoction on lung function of diabetic rats with deficiency of qi and Yin. Methods: Six male rats of SPF grade SD at 6 weeks old were randomly selected as the control group, and the remaining 54 rats were prepared by high-fat and high-sugar feeding combined with one-time intraperitoneal injection of streptozotocin (STZ) 30mg/kg dissolved in 0.1mol /L sterile sodium citrate buffer (pH 4.0). Choose 30 diabetic rats with Qi and Yin deficiency were randomly divided into 5 groups: model group, Baihu and ginseng decoction high dose group, medium dose group, low dose group, metformin group, 6 rats in each group. High-dose, medium-dose and low-dose groups were given 27.5g•kg-1•d-1, 13.8g•kg-1•d-1 and 6.9g•kg-1•d-1 Baihu and ginseng decoction respectively for gavage, and continuous administration was conducted for 3 weeks. After 3 weeks of treatment, the effects of different doses of Baihu and ginseng decoction on blood glucose, body weight, general condition and pulmonary function parameters of DM rats were observed.Results: Compared with the model group, high, medium and low doses of baihu and ginseng decoction can improve the pulmonary function parameters of DM rats, such as forced vital capacity (FVC), instantaneous velocity of late respiration (FEF25% or V25) and peak inspiratory velocity (PIF). Conclusion: Baihu and ginseng decoction can reduce the blood glucose of DM rats, and significantly improve the pulmonary function of DM rats with deficiency of Qi and Yin, especially with high dose.


   国内外研究证实,肺亦是糖尿病(Diabetes mellitus,DM)的靶器官[1],临床多表现为不同程度的肺通气功能和弥散功能下降[2],但其发病机制尚未明确,亦未提出明确的治疗方法,严重危害了人们的生命健康。DM肺损伤大致类似于中医学“上消”“消渴”“肺消”等范畴,明代医家孙志宏在《简明医彀•三消》明确提出“上焦受病,多饮而少食,小便多利而不禁,甚至舌干白苔或裂,属肺,名消渴,亦名膈消。”其基本病机是热盛伤津,导致气阴两虚[3],古今医家临床多选用清热养阴益气的白虎加人参汤辨证治疗气阴两虚型消渴,效果显著,但该方对于DM肺功能的影响仍处于空白阶段。本研究通过复制DM模型大鼠,探讨白虎加人参汤对DM肺功能的影响,以期为临床防治DM肺损伤提供理论支撑和研究基础。


   参考文献

[1] Ali MO: Pulmonary complications in diabetes mellitus. [2]Mekov EV, Slavova YG, Genova MP, Tsakova AD, Kostadinov DT, Minchev DD, Marinova DM, Boyanov MA: Diabetes Mellitus Type 2 in Hospitalized COPD Patients: Impact on Quality of Life and Lung Function. Folia medica. 2016, 58(1):36-41.

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[4] 吴晏,韩静,黄黎明,郭淑贞,王伟.2型糖尿病模型大鼠表征及其证候动态演变[J].中华中医药杂志,2011,26(11)2533-2537.

[5]Girish Talakatta, Mohsen Sarikhani, Jaseer Muhamed, K. Dhanya, Bagganahalli S. Somashekar, Padukudru Anand Mahesh, Nagalingam Sundaresan, P. V. Ravindra. Diabetes induces fibrotic changes in the lung through the activation of TGF-β signaling pathways. Scientific Reports. 2018,8(1):11920.

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[7]武志娟,刘敏.刘敏运用经方治疗糖尿病经验[J].广州中医药大学学报,2019,36(02):281-284.

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 白虎加人参汤对气阴两虚型糖尿病模型大鼠肺功能的影响.pdf


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